VICI M50 - Priming and Testing the Pump; Tubing Connections (M6 Shown); Initial Priming

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Priming and Testing the Pump
At this stage, some basic tests will determine that the tubing connections are
tight and that the pump is rotating in the proper direction. The tests include an
initial priming of the pump to remove all air and unwanted solutions in the tubing.
Use proper safety precautions when pumping flammable
liquids. In all applications, make sure you have enough
tubing and sufficient quantities of solutions ready for
use before beginning a procedure.
Tubing Connections (M6 shown)
M6pumpsshouldnotbeusedwithtubingODssmallerthan1/16".ForM50
pumps,theminimumODis1/8".
Improperly installed fittings can create space for a
bubble to become trapped, degrading pump performance.
The use of non-VICI fittings can also adversely affect
pump function and performance.
1. Connectalengthoftubingtotheinlet(PortA)andplacetheotherendin
a vessel containing isopropyl alcohol.
2. Connectasecondlengthoftubingtotheoutlet(PortB)andplacethe
other end into a receiver bottle.
Fitting Assembly Instructions
1. Slidethenutandferruleonto
the tubing in the order shown.
2. Insert this assembly into the
fitting detail, screwing the nut in
two or three turns by hand.
3. Pushthetubingallthewayforwardintothedetailsothatitseatsrmly.
This is essential for a proper zero dead volume connection.
4. Turn the nut into the detail until it is finger tight.
Initial Priming
In the Test Mode, a mouse click on a button directs the pump to either
aspirate or dispense. (Refer to page 13, No. 7 for a definition, if required.)
WhentheEnableboxintheFixedVolumeareaischecked,(seeFigure 8)
thepumpwillaspirateordispensethequantityenteredintheFixedVolume
field and then stop. If the Enable box is not selected, the pump will continue
to run at the set Flow Rate until either Stop (stops aspirating or dispensing)
orQuit(exitstheTestMode)isselected.
If the pump aspirates a volume larger than the outlet
tube volume, sample or reagent could enter the pump
and mix with liquid in the reservoir.