30 HDR-DAD Solution
3
Installation of the HDR-DAD Solution
Calculation of the Delay Volume
Calculation of the Delay Volume
No shipment of caffeine standard to China.
1 Install Restriction capillary, SST 0.12 mm ID, 2 m long (5022-2159) in place
of the column.
2 Inject 1 μL of Caffeine in Water, 10 mg/L, 50 mL (5063-6524) at a moderate
flow rate (for example 0.5 μL/min).
Parts required # p/n Description
1 5022-2159 Restriction capillary, SST 0.12 mm ID, 2 m long
1 5063-6524 Caffeine in Water, 10 mg/L, 50 mL
IQ test sample, for UV/VIS spectrophotometer
Suggestion:
For statistical calculations use 6 sample injections.
The amount of sample is selected to run both detectors in linear range (for example
50 µg/mL, V
inj
= 1µL => 50 ... 100mAU/cm).
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