Getting Started
Step 3. Prepare the Triple Quad instrument
16 Agilent 6400 Series Triple Quadrupole LC/MS Quick Start Guide
Step 3. Prepare the Triple Quad instrument
Do a Checktune, Autotune or Manual Tune
1 From the Context list, click Tune.
You can see the Instrument Status window, the Actuals window and the Tune
window when you switch to the Tune context. Click Tune in the View menu if
the Tune window is not visible.
2 Click Checktune to evaluate if the MS parameters are within the limits to
produce the specified mass accuracy and resolution. Checktune takes
approximately 3 minutes to run for each polarity.
If the current tune file was last tuned with the Fast Scan Autotune, then the
Checktune algorithm only checks MS2. If the current tune file was last tuned
with Autotune, then the Checktune algorithm checks both MS1 and MS2.
If the current tune file was last tuned with the Fast Scan Autotune, then the
Fast Scan check box is marked on the Manual Tune tab.
Do a Checktune regularly.
You can run a Checktune with the following sources: ESI, AJS ESI, MMI and
HPLC-Chip.
If Checktune passes, then skip to step 5.
If Checktune fails, then you can try using the Manual Tune tab to fix the
problem. See the next step.
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