Exercise 3 – Create a Triggered Dynamic MRM acquisition method
Task 2. Add/Modify compounds in an existing database
Agilent 6400 Series Triple Quad LC/MS Familiarization Guide 51
13 Review the Primary transitions and
Trigger transitions.
• For sulfachloropyridazine, select
285 m/z -> 155.7 m/z transition
as the Primary and Trigger
transition.
• For sulfadimethoxine, select 311
m/z -> 155.7 m/z transition as
the Primary and Trigger
transition.
• For sulfamethazine, select 279
m/z -> 185.7 m/z transition as
the Primary and Trigger
transition.
• For sulfamethizole, select 271
m/z -> 155.8 m/z transition as
the Primary and Trigger
transition.
• Review each compound. Change the
Primary and Trigger transitions to the
transitions listed in the left column.
• Change the other Primary transitions
as shown below.
• The program selected the most
abundant transitions which in this
example often had a low m/z for
the Product Ion. A very abundant
low m/z ion may be unsuitable as a
Primary transition.
• You can select two Primary
transitions as triggers for a
compound.
• In the example below, all of the
columns have values. The
Fragmentor voltages and collision
energy values were set in
Optimizer.
• See Table 5 on page 54 for values to
use in the method.
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