Exercise 3 – Create a Triggered Dynamic MRM acquisition method
Task 3. Create a Triggered Dynamic MRM method from an existing database
Agilent 6400 Series Triple Quad LC/MS Familiarization Guide 57
3 Review the method in the Dynamic
MRM Viewer dialog box.
a Right-click the Scan segments table
and click Edit DMRM Method. The
Dynamic MRM Viewer dialog box is
opened.
b Type
200 for the Cycle time. This
value is shown in the Acquisition tab.
c Click between the Primaries only
button and the All transitions button
if the Dynamic MRM Statistics
information is not updating. Then,
click the All transitions button.
d Click Close.
• The compounds in Optimizer were
overwritten by the compounds that
you updated in the Database
Browser program.
• You can modify the Cycle time and
see how the Minimum Dwell Time
is changed. If the Minimum Dwell
Time is less than 5 ms, and
especially if it is less than 2 ms,
then signal-to-noise is poor.
• A Dwell Time of 8 ms per transition
is fine.
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