50 Agilent 1200 Preparative Pump User Manual
3 Installing the Pump
Get the System Ready for the First Analysis
5 When the pumping system has been turned OFF for a certain time (for
example, overnight) repeat Step 3 (step 4 on page 49).
Tabl e 10 Choice of Priming Solvents for Different Purposes
Activity Solvent Comments
• After an installation
• When switching between
reverse phase and normal
phase (both times)
Isopropanol Best solvent to flush air out of the
system
• After an installation Ethanol or Methanol Alternative to Isopropanol (second
choice) if no Isopropanol is available
• To clean the system when
using buffers
• After a solvent change
Bidistilled water Best solvent to re-dissolve salts
• After the installation of
normal phase solvents
Hexane + 5% Isopropanol Good wetting properties
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