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Applied Biosystems GeneAmp PCR System 2700 User Manual

Applied Biosystems GeneAmp PCR System 2700
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Applied Biosystems 2720
Thermal Cycler
For Amplification of Nucleic Acids
User Guide

Table of Contents

Questions and Answers:

Applied Biosystems GeneAmp PCR System 2700 Specifications

General IconGeneral
Temperature Range4°C to 99°C
Temperature Uniformity±0.5°C
Block Capacity96 x 0.2 mL tubes
DisplayLCD
InterfaceRS-232
Temperature Accuracy±0.25°C (35-99.9°C)
Thermal Cycler TypePeltier-based

Summary

Safety and EMC Compliance Information

Safety Conventions Used in This Document

Defines alert words (IMPORTANT, CAUTION, WARNING, DANGER) and their symbols.

General Instrument Safety

Covers safety for moving, lifting, and operating the instrument.

Chemical Safety

Provides warnings and guidelines for handling chemicals safely.

Chemical Waste Safety

Details safety guidelines for handling and disposing of chemical waste.

Electrical Safety

Covers safety precautions related to grounding, fuses, power, and overvoltage.

Physical Hazard Safety

Warns about hazards from moving parts and hot surfaces.

System Overview

Introducing the Applied Biosystems 2720 Thermal Cycler

Describes the instrument, its features, and specifications.

About the Control Panel

Explains the control panel's components: screen, keys, and navigation.

Getting Started

Powering On

Provides step-by-step instructions to turn on the instrument.

Adding Yourself as a User

Explains how to add a user name for method separation and storage.

Editing Your Method

Instructs on how to modify an existing method's parameters.

Starting and Stopping a Run

Details procedures to start and stop a PCR run.

Runs

Loading Samples

Provides procedures for loading samples with different tube configurations.

Starting a Run

Step-by-step guide to initiating a PCR run on the instrument.

Pausing or Stopping a Run

Explains how to pause a run and how to stop it completely.

Methods and Users

Adding, Changing, or Deleting a User Name

Covers adding, modifying, or removing user accounts on the system.

Creating a Method

Details the graphical representation of methods on the Create/Edit screen.

Changing a Method Using Advanced Features

Explains how to modify methods using advanced features like holds and cycles.

Utilities

Configuring the Instrument

Explains how to set instrument parameters like time, date, and printer settings.

Running Hardware Diagnostics

Covers performing display and keypad diagnostic tests.

Running System Performance Diagnostics

Details running Rate and Cycle tests to check heating/cooling systems.

Maintenance

Cleaning the Instrument

Provides instructions for cleaning the sample wells and heated cover.

Replacing Fuses

Step-by-step guide on how to safely replace instrument fuses.

Troubleshooting

Display Screen Error Messages

Lists error messages, their descriptions, and recommended actions.

Serious Error Messages

Lists error messages indicating potential hardware malfunctions and their solutions.

Troubleshooting Information

Provides a table of common problems, causes, and remedies.

Specifications

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