ViiA™ 7 Real-Time PCR System
QUICK REFERENCE GUIDE
Note: For safety and biohazard guidelines, refer to the
“Safety” appendix in the Applied Biosystems ViiA
™
7Real-
Time PCR System User Guide (PN 4442661). For every
chemical, read the SDS and follow the handling
instructions. Wear appropriate protective eyewear, clothing,
and gloves.
In this guide
■ Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
■ Calibrate the ViiA
™
7 System . . . . . . . . . . . . . . . . . . . . . . . . . . 2
■ Perform an experiment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
■ Maintain the ViiA
™
7 System. . . . . . . . . . . . . . . . . . . . . . . . . . 15
■ Power off the ViiA
™
7 System. . . . . . . . . . . . . . . . . . . . . . . . . 22
For details on operating and maintaining your ViiA
™
7 System, refer
to the Applied Biosystems ViiA
™
7 Real-Time PCR System User Guide
(PN 4442661).
Description
The Applied Biosystems ViiA
™
7 Real-Time PCR System uses
fluorescent-based polymerase chain reaction (PCR) reagents to
provide:
• Quantitative research detection of target nucleic acid
sequences (targets) using real-time analysis.
• Qualitative research detection of targets using post-PCR
(endpoint) analysis.
• Qualitative analysis of the PCR product achieved by melt curve
analysis that occurs post-PCR.
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