11
Perform an experiment
Applied Biosystems ViiA
™
7 Real-Time PCR System Quick Reference Guide
4
Centrifuge the array
card (continued)
b. Place the filled carrier clips into the centrifuge buckets. Make sure that
the array-card fill reservoirs and bucket and clip labels face outward
when loaded into the centrifuge.
You must run the centrifuge with all four buckets in place and each of
the two carriers filled with array cards. Place empty array cards into
unfilled slots.
IMPORTANT! Balance the loads in opposite buckets in the centrifuge.
c. Close the centrifuge cover, then centrifuge the array card for 1 minute at 1200 rpm. When the
run is finished, stop the centrifuge, then centrifuge the array card again for 1 minute at 1200 rpm.
IMPORTANT! Do not try to save time by doing one spin for 2 minutes. Centrifuging twice is
important for a good fill into the array card.
d. Open the centrifuge and check that the fluid levels in the reservoirs of the array card have
decreased by the same amount. Also, check for bubbles in all wells.
If necessary, centrifuge the array card for an additional minute to fill unfilled wells. Do not exceed
three 1-minute runs or centrifuge the array card for longer than 1 minute at a time.
5
Seal the array card a. With the carriage (roller assembly) of the staker/sealer
fixture in the Start position, place a filled array card into the
fixture with the foil side up so that the fill reservoirs are the
farthest away from the carriage.
b. Press down on all four corners of the array card to ensure
that it is fully seated within the fixture.
c. Use the two alignment pins in the fixture to position the
array card correctly.
Correct Incorrect
GR2172a
Alignment pins
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