5
Perform an experiment
Applied Biosystems ViiA
™
7 Real-Time PCR System Quick Reference Guide
Perform an experiment
Materials required
• For all sample blocks:
– DI water/ DEPC water
–Microcentrifuge tubes
– Pipettors and pipette tips
–Vortex mixer
–Safety goggles
– Powder-free gloves
– Sample stock
– Standard stock
– Reaction mix components
• For 384-well, 96-well, or Fast 96-well sample blocks:
– Centrifuge with plate adapter
– Plate or (96-well and Fast 96-well blocks only) tube strip, or single tube
– Optical adhesive film
• For array card sample blocks:
– Centrifuge with centrifuge array-card carrier clips and buckets
– Array-card staker/sealer fixture
–Array card
Workflow
Design the experiment (page 6)
Prepare the reactions (page 8)
Start and monitor the experiment (page 12)
View analysis reports (page 13)
Export results (page 14)