Applied Biosystems ViiA 7 Real User Manual

2
Calibrate the ViiA
™
7 System
Applied Biosystems ViiA
™
7 Real-Time PCR System Quick Reference Guide
Calibrate the ViiA
™
7 System
Calibration types
• Regions of interest (ROI)
•Background
•Uniformity
• Dye (only for dyes used in your experiments)
• Normalization
• Instrument verification
Materials required
384-well sample block
Array-card sample block
96-well sample block
• 384-Well Spectral Calibration Plate with FAM
™
Dye (PN 4432271)
• 384-Well Spectral Calibration Plate with VIC
®
Dye (PN 4432278)
• 384-Well Spectral Calibration Plate with ROX
™
Dye (PN 4432284)
• 384-Well Spectral Calibration Plate with SYBR
®
Green Dye (PN 4432290)
• 384-Well Spectral Calibration Plate with TAMRA
™
Dye (PN 4432296)
• 384-Well Spectral Calibration Plate with NED
™
Dye (PN 4432302)
• 384-Well Region of Interest (ROI) and Background Plates (PN 4432320)
• 384-Well Normalization Plates with FAM
™
/ROX
™
and VIC
®
/ROX
™
Dyes
(PN 4432308)
• TaqMan RNase P 384-Well Instrument Verification Plate (PN 4455280)
•ViiA
™
7 System 384-Well Plate Adapter
(PN 4457087)
• Centrifuge with plate adapter
• Powder-free gloves
• Safety goggles
• Array Card Spectral Calibration Dye Kit (PN 4432314)
•TaqMan
®
RNase P Array Card Instrument Verification Reagents Kit
(PN 4432265)
•ViiA
™
7 System Array Card Adapter
(PN 4454166)
• Centrifuge with centrifuge array-card carrier
clips and buckets
• Powder-free gloves
• Safety goggles
• 200-µL pipettor, with pipette tips
• Array-card staker/sealer fixture
• 96-Well Spectral Calibration Plate with FAM
™
Dye (PN 4432327)
• 96-Well Spectral Calibration Plate with VIC
®
Dye (PN 4432334)
• 96-Well Spectral Calibration Plate with ROX
™
Dye (PN 4432340)
• 96-Well Spectral Calibration Plate with SYBR
®
Green Dye (PN 4432346)
• 96-Well Spectral Calibration Plate with TAMRA
™
Dye (PN 4432352)
• 96-Well Spectral Calibration Plate with NED
™
Dye (PN 4432358)
• 96-Well Region of Interest (ROI) and Background Plates (PN 4432364)
• 96-Well Normalization Plates with FAM
™
/ROX
™
and VIC
®
/ROX
™
Dyes
(PN 4432370)
•TaqMan
®
RNase P 96-Well Instrument Verification Plate (PN 4432382)
•ViiA
™
7 System 96-Well Plate Adapter (PN
4459845)
• Centrifuge with plate adapter
• Powder-free gloves
• Safety goggles

Applied Biosystems ViiA 7 Real Specifications

General

Brand	Applied Biosystems
Model	ViiA 7 Real
Category	Laboratory Equipment
Language	English