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Applied Biosystems ViiA 7 Real User Manual

Applied Biosystems ViiA 7 Real
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Calibrate the ViiA
7 System
Applied Biosystems ViiA
7 Real-Time PCR System Quick Reference Guide
Calibrate the ViiA
7 System
Calibration types
Regions of interest (ROI)
•Background
•Uniformity
Dye (only for dyes used in your experiments)
Normalization
Instrument verification
Materials required
384-well sample block
Array-card sample block
96-well sample block
384-Well Spectral Calibration Plate with FAM
Dye (PN 4432271)
384-Well Spectral Calibration Plate with VIC
®
Dye (PN 4432278)
384-Well Spectral Calibration Plate with ROX
Dye (PN 4432284)
384-Well Spectral Calibration Plate with SYBR
®
Green Dye (PN 4432290)
384-Well Spectral Calibration Plate with TAMRA
Dye (PN 4432296)
384-Well Spectral Calibration Plate with NED
Dye (PN 4432302)
384-Well Region of Interest (ROI) and Background Plates (PN 4432320)
384-Well Normalization Plates with FAM
/ROX
and VIC
®
/ROX
Dyes
(PN 4432308)
TaqMan RNase P 384-Well Instrument Verification Plate (PN 4455280)
•ViiA
7 System 384-Well Plate Adapter
(PN 4457087)
Centrifuge with plate adapter
Powder-free gloves
Safety goggles
Array Card Spectral Calibration Dye Kit (PN 4432314)
•TaqMan
®
RNase P Array Card Instrument Verification Reagents Kit
(PN 4432265)
•ViiA
7 System Array Card Adapter
(PN 4454166)
Centrifuge with centrifuge array-card carrier
clips and buckets
Powder-free gloves
Safety goggles
200-µL pipettor, with pipette tips
Array-card staker/sealer fixture
96-Well Spectral Calibration Plate with FAM
Dye (PN 4432327)
96-Well Spectral Calibration Plate with VIC
®
Dye (PN 4432334)
96-Well Spectral Calibration Plate with ROX
Dye (PN 4432340)
96-Well Spectral Calibration Plate with SYBR
®
Green Dye (PN 4432346)
96-Well Spectral Calibration Plate with TAMRA
Dye (PN 4432352)
96-Well Spectral Calibration Plate with NED
Dye (PN 4432358)
96-Well Region of Interest (ROI) and Background Plates (PN 4432364)
96-Well Normalization Plates with FAM
/ROX
and VIC
®
/ROX
Dyes
(PN 4432370)
•TaqMan
®
RNase P 96-Well Instrument Verification Plate (PN 4432382)
•ViiA
7 System 96-Well Plate Adapter (PN
4459845)
Centrifuge with plate adapter
Powder-free gloves
Safety goggles

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Applied Biosystems ViiA 7 Real Specifications

General IconGeneral
BrandApplied Biosystems
ModelViiA 7 Real
CategoryLaboratory Equipment
LanguageEnglish

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