Applied Biosystems ViiA 7 Real User Manual

10
Perform an experiment
Applied Biosystems ViiA
™
7 Real-Time PCR System Quick Reference Guide
Prepare the array card
IMPORTANT! Wear powder-free gloves when you handle the plate or array card.
Note: Visit www2.appliedbiosystems.com/lib/multimedia/taqman_tlda/tlda_1.cfm to view an online video of loading, centrifuging, and
sealing an array card.
1
Remove the array card Remove the Applied Biosystems Array Card from the box and place it on a clean, dry surface.
2
Load chemicals and
solutions into the array
card port
For each chemical and solution transferred:
a. Place the array card on a lab bench, with the foil side down.
b. Load 100 µL of fluid into a pipette.
c. Hold the pipette at an angle (~45 degrees) and place the tip into the fill port.
Note: The fill port is the larger of the two holes on the left arm of each fill reservoir.
IMPORTANT! Do not allow the tip to contact and possibly damage the coated foil beneath the
fill port.
3
Pipette fluid into the fill
reservoir
Dispense the fluid so that it sweeps in and around the fill reservoir
toward the vent port. Pipette fluid into the fill reservoir, but do not go
past the first stop of pipettor plunger when pipetting the reagents into
the array card, or you may blow the solution out of the port.
IMPORTANT! Do not allow the tip to contact and possibly damage the
coated foil beneath the fill port.
4
Centrifuge the array
card
a. Place the filled array card into a centrifuge array-card carrier clip
and place empty array cards in the remaining slots. Make sure
that the labels on the buckets and clips face the same way.
IMPORTANT! Balance the loads in opposite buckets in the
centrifuge.
Fill
port
Vent
port
F
i
lled array
Empty
array

Applied Biosystems ViiA 7 Real Specifications

General

Brand	Applied Biosystems
Model	ViiA 7 Real
Category	Laboratory Equipment
Language	English