To Next PageTo Next Page
Need help?Do you have a question about the Applied Biosystems 7500 Fast and is the answer not in the manual?
General| Instrument Type | Real-Time PCR System |
|---|---|
| Temperature Uniformity | ±0.5 °C |
| Heating Rate | 4.4 °C/sec |
| Ramp Rate (heating) | 4.4 °C/sec |
| Detection Channels | 5 |
| Data Acquisition | Continuous |
| Thermal Cycler Block | 96-well |
| Sample Capacity | 96-well |
| Format | 96-well plate |
| Reaction Volume | 10–100 μL |
| Temperature Range | 4°C to 100°C |
| Temperature Accuracy | ±0.25 °C (35–100 °C) |
| Detection Method | Fluorescence |
| Excitation Source | LED |
| Excitation Wavelengths | 470 nm, 520 nm, 580 nm, 623 nm |
| Dyes | SYBR Green, FAM, VIC, NED, ROX, Texas Red |
| Power Requirements | 100–240 VAC, 50/60 Hz |
Covers essential safety practices for instrument operation and handling.
Details procedures and precautions for handling chemicals safely.
Guidelines for the proper and safe disposal of chemical waste.
Focuses on electrical hazards, including power supply and fuse safety.
Addresses safety related to moving parts, power, and physical risks.
Outlines the recommended workflow and time estimates for system installation.
Detailed steps for safely unpacking and positioning the instrument.
Step-by-step guide for physically connecting and powering on the instrument.
Detailed procedure for installing the SDS software package.
Instructions for connecting the instrument and powering it on.
Guide to running and interpreting the instrument's function test.
Steps for preparing the specific calibration plate for ROI.
Detailed procedure for executing the ROI calibration on the instrument.
Instructions for preparing the background calibration plate.
Procedure for running the background calibration.
Guide to analyzing the data from the background calibration.
Procedure for optical calibration specific to 7500/7500 Fast systems.
Solutions for issues encountered during RNase P plate runs.
