Applied Biosystems 7500 Fast User Manual

Chapter 3 Installing the Software and Powering On the Instrument
Overview
24 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide
Notes
Overview
Note: You can also use the Real-Time System Setup wizard to perform the procedures in
this guide. To access the wizard, insert the installation CD in the CD drive of the
computer. The Real-Time System Setup wizard starts automatically after a slight delay
when you insert the CD.
Time Required 45 minutes
Materials
Required
IMPORTANT! If you plan to configure the 7300/7500/7500 Fast system computer with
additional software (see page 10), complete the installation of the 7300/7500/7500 Fast
system as described in this guide before installing other software. Installing third-party
software to the 7300/7500/7500 Fast system computer before completing the procedures
in this manual can complicate the installation.
SDS Software and
Documentation CDs
Powder-free gloves
Safety glasses

Applied Biosystems 7500 Fast Specifications

General

Instrument Type	Real-Time PCR System
Temperature Uniformity	±0.5 °C
Heating Rate	4.4 °C/sec
Ramp Rate (heating)	4.4 °C/sec
Detection Channels	5
Data Acquisition	Continuous
Thermal Cycler Block	96-well
Sample Capacity	96-well
Format	96-well plate
Reaction Volume	10–100 μL
Temperature Range	4°C to 100°C
Temperature Accuracy	±0.25 °C (35–100 °C)
Detection Method	Fluorescence
Excitation Source	LED
Excitation Wavelengths	470 nm, 520 nm, 580 nm, 623 nm
Dyes	SYBR Green, FAM, VIC, NED, ROX, Texas Red
Power Requirements	100–240 VAC, 50/60 Hz