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Applied Biosystems 2720 User Manual

Applied Biosystems 2720
122 pages
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Applied Biosystems 2720
Thermal Cycler
For Amplification of Nucleic Acids
User Guide

Table of Contents

Questions and Answers:

Applied Biosystems 2720 Specifications

General IconGeneral
TypeThermal Cycler
Temperature Accuracy±0.25°C
Temperature Uniformity±0.5°C
DisplayLCD
Power100-240V

Summary

Preface

How to Use This Guide

Explains how to use the manual, including text conventions and user attention words.

How to Obtain More Information

Provides details on related documentation and how to contact the company.

How to Obtain Support

Guides users on how to find support services and contact information.

Safety and EMC Compliance Information

Safety Conventions Used in This Document

Defines safety alert words (IMPORTANT, CAUTION, WARNING, DANGER) and symbols.

Symbols on Instruments

Describes electrical and safety symbols used on Applied Biosystems instruments.

Safety Labels on Instruments

Lists CAUTION, WARNING, and DANGER statements found on instrument labels.

Instrument Warnings Diagram

Shows the location of hazard and warning labels on the instrument's rear.

General Instrument Safety

Covers safety precautions for moving, lifting, and operating the instrument.

Chemical Safety

Details hazards and precautions related to handling chemicals.

Chemical Waste Safety

Provides guidelines for safely handling and disposing of chemical waste.

Electrical Safety

Outlines safety measures related to grounding, fuses, power, and overvoltage.

Laboratory Environmental Requirements

Specifies conditions for safe operation and storage of the instrument.

Physical Hazard Safety

Warns about hazards from moving parts and hot surfaces.

Safety and Electromagnetic Compatibility (EMC) Standards

Lists compliance with various safety and EMC standards.

System Overview

Overview

Introduces the chapter and its topics.

Introducing the Applied Biosystems 2720 Thermal Cycler

Describes the instrument's purpose, features, and comparison to previous models.

About the Control Panel

Details the instrument's control panel layout and functions.

Overview of Functions

Summarizes the main functions accessible from the Main Menu.

Introducing an Important Screen

Explains the graphical representation of a method on the Create/Edit screen.

Getting Started

Overview

Introduces the chapter and its basic procedures.

Powering On

Provides step-by-step instructions to turn on the instrument.

Adding Yourself as a User

Guides users on how to create a user profile for method management.

Creating a Method

Explains how to create and save a new PCR method.

Editing Your Method

Details the process of modifying an existing stored method.

Starting and Stopping a Run

Describes how to initiate and then abort a PCR run.

More Features

Introduces additional system features beyond basic operation.

Runs

Overview

Explains how to run samples, use disposables, and manage runs.

Using Disposables

Describes the different types of MicroAmp disposables available.

Loading Samples

Provides procedures for loading samples into tubes or plates.

Starting a Run

Details the steps to start a PCR run from the Main Menu.

Pausing or Stopping a Run

Explains how to pause, resume, or completely stop a run.

Reviewing the History of a Run

Guides on how to access and interpret run history data.

When a Run Completes

Describes what happens when a run finishes or is aborted.

Methods and Users

Overview

Covers creating, editing, and managing methods and user names.

Adding, Changing, or Deleting a User Name

Details procedures for managing user accounts on the instrument.

Creating a Method

Explains how to create and store a new PCR method.

Changing a Method Using Advanced Features

Describes how to edit methods with advanced options like holds and cycles.

Handling Methods

Explains how to select, sort, search, print, and delete methods.

Utilities

Overview

Introduces functions available from the Utilities menu.

Configuring the Instrument

Explains how to set instrument parameters like time, date, and printer.

Upgrading System Firmware

Provides instructions for updating the instrument's firmware.

Connecting to a Printer

Details how to connect an optional printer to the instrument.

Calculating the Melting Temperature

Guides on using the Tm Calculator for primer analysis.

Running Hardware Diagnostics

Explains how to perform display and keypad diagnostic tests.

Running the Calibration Verification Test

Describes the test for verifying instrument temperature calibration.

Running the Temperature Non-Uniformity Test

Explains the test for sample block temperature uniformity.

Running System Performance Diagnostics

Guides on performing Rate and Cycle tests for system performance.

Maintenance

Overview

Introduces procedures for maintaining the thermal cycler.

Cleaning the Instrument

Provides instructions for cleaning the instrument's sample wells and cover.

Replacing Fuses

Details the steps for safely replacing instrument fuses.

Troubleshooting

Overview

Explains how to diagnose and solve instrument problems.

If There’s a Power Failure

Describes the instrument's behavior during power outages.

Display Screen Error Messages

Lists error messages, their causes, and recommended actions.

Troubleshooting Information

Provides a table of common problems, causes, and remedies.

Specifications

Overview

Introduces the appendix detailing instrument specifications.

System Specifications

Lists dimensions, weight, power requirements, and configurations.

Control Panel Specifications

Details specifications for the display screen and control panel keys.

Sample Temperature Information

Provides information on sample temperature range and calibration.

Printer Specifications

Lists serial interface board specifications for printer connectivity.

Supplied Methods

Overview

Introduces the appendix describing precoded methods.

About the Methods

Lists and briefly describes the five precoded methods available.

Screen Flowcharts

Overview

Introduces flowcharts for understanding procedures.

Run

Provides a flowchart for the instrument's Run function.

Stop or End of Run

Shows a flowchart for stopping or ending a run.

Create/Edit

Provides a flowchart for creating and editing methods.

User

Displays a flowchart for managing user accounts.

Utilities

Presents a flowchart for accessing instrument utilities.

Diagnostics

Shows a flowchart for running instrument diagnostics.

Upgrade

Provides a flowchart for firmware upgrades.

Instrument Warranty Information

Overview

Introduces information on warranty, configuration, and claims.

Computer Configuration

Discusses recommended computer configurations for the instrument.

Limited Product Warranty

Details the warranty terms for instruments and accessories.

Damages, Claims, and Returns

Outlines procedures for handling product damage, claims, and returns.

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