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StepOne
Applied Biosystems StepOne User Manual
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Applied
Biosystems StepOne
™
and StepOnePlus
™
Real-T
ime PCR Systems
Installation, Networking, and Maintenance
Installation, Networking
, and Maintenance Guide
2
Table of Contents
Default Chapter
5
Table of Contents
5
Preface
9
Purpose of this Guide
9
How to Obtain more Information
11
How to Obtain Support
12
Safety Conventions Used in this Document
13
Symbols on the Instruments
14
Safety Labels on Instruments
15
General Instrument Safety
16
Chemical Safety
17
Chemical Waste Safety
19
Electrical Safety
20
LED Safety
20
Biological Hazard Safety
21
Workstation Safety
21
Safety and Electromagnetic Compatibility (EMC) Standards
22
Chapter 1 Get Started
23
Get Started
23
About the Stepone and Steponeplus Systems
24
About the Stepone ™ and Steponeplus
24
Systems
24
Before You Begin
27
Select Protective Hardware and Software
29
Select an Installation Layout
31
Plan the Installation
33
Chapter 2 Set up the Instrument
35
Real-Time PCR System Software
35
About the Installation
36
Unpack the Instrument
37
Check the Shipped Materials
39
Set up the Instrument
42
Complete the Installation
45
Chapter 3 Install the Colocated Layout
47
About the Colocated Installation
48
Install the Computer
49
Set up the Computer
50
Install the Stepone Software
53
Install the Stepone ™ Software
53
Connect the System Components
57
Perform the Rnase P Experiment
60
Set up the Experiment
62
Run the Experiment
63
Analyze the Experiment
65
After the Installation
69
Chapter 4 Install the Standalone Layout
71
About the Standalone Installation
72
Set up the Computer
73
Install the Computer
74
Install the Stepone Software
76
Install the Stepone ™ Software
76
Prepare the Stepone ™ Software for Standalone Use
80
Perform the Rnase P Experiment
82
Set up the Experiment
84
Run the Experiment
85
Analyze the Rnase P Experiment
87
Unload the Instrument
87
Transfer the Experiment
88
Analyze the Experiment
90
After the Installation
93
Chapter 5 Connect the System to a Network
95
Help from Within the Applied Biosystems Stepone
95
By Pressing F1, or Clicking
95
Overview
96
Connect the Instrument to a Network
100
Set up a Computer for Remote Monitoring
102
Monitor the Instrument
104
Chapter 6 Maintain the System
109
Regular Maintenance
110
Perform a Spatial Calibration
112
Perform a Background Calibration
116
Perform a Dye Calibration
122
Archive and Back up Data
135
Infrequent Maintenance
136
Decontaminate the Sample Block
137
Decontaminate the Sample Block(S)
137
Move the Instrument
140
Replace the External Fuses
142
Ship the Instrument for Service
144
Update the Windows Operating System
146
Update the Stepone Software
147
Update the Stepone
147
Appendix A How to Troubleshoot the Installation
149
Stepone ™ Software Problems
150
Network Connection Problems
151
Background Calibration Failure
154
Dye Calibration Failure
156
Glossary
159
Index
175
4
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Applied Biosystems StepOne Specifications
General
Brand
Applied Biosystems
Model
StepOne
Category
Laboratory Equipment
Language
English
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