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Applied Biosystems StepOne User Manual

Applied Biosystems StepOne
184 pages
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Applied Biosystems StepOne
and StepOnePlus
Real-Time PCR Systems
Installation, Networking, and Maintenance
Installation, Networking, and Maintenance Guide

Table of Contents

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Applied Biosystems StepOne Specifications

General IconGeneral
BrandApplied Biosystems
ModelStepOne
CategoryLaboratory Equipment
LanguageEnglish

Summary

Get Started

About the StepOne and StepOnePlus Systems

Provides details on the two available models of the Real-Time PCR System.

Before You Begin

Outlines guide usage, installation preparation, and required materials.

Select Protective Hardware and Software

Recommends protective devices and software for optimal performance.

Select an Installation Layout

Describes two installation layouts: standalone and colocated.

Plan the Installation

Presents a recommended workflow for installing the system.

Set Up the Instrument

About the Installation

Explains the installation process for both colocated and standalone layouts.

Unpack the Instrument

Details site requirements and procedures for unpacking the instrument.

Check the Shipped Materials

Lists components to check after unpacking the instrument and packing kit.

Set Up the Instrument

Guides on connecting power, optional protective devices, and initial diagnostics.

Complete the Installation

Instructs on completing remaining components and performing RNase P verification.

Install the Colocated Layout

About the Colocated Installation

Explains when to use the colocated layout and its connection method.

Install the Computer

Covers unpacking, setting up, connecting computer components, and powering on.

Install the StepOne Software

Details how to prepare the OS and install the StepOne software.

Connect the System Components

Guides on establishing and confirming the connection between instrument and computer.

Perform the RNase P Experiment

Details how to set up, run, and analyze the RNase P verification experiment.

After the Installation

Covers installing additional software and connecting to a network.

Install the Standalone Layout

About the Standalone Installation

Explains when to use the standalone layout and its data transfer method.

Set Up the Computer

Covers unpacking, setting up, connecting computer components, and powering on.

Install the StepOne Software

Details how to prepare the OS and install the StepOne software.

Prepare the StepOne Software for Standalone Use

Sets software preferences to avoid instrument connection attempts.

Perform the RNase P Experiment

Details how to set up, run, and analyze the RNase P verification experiment.

Analyze the RNase P Experiment

Covers unloading, transferring, and analyzing the RNase P experiment data.

After the Installation

Covers installing additional software and connecting to a network.

Connect the System to a Network

Overview

Explains how connecting to a network enhances instrument functionality.

Connect the Instrument to a Network

Guides on setting up instrument network connection according to policies.

Set Up a Computer for Remote Monitoring

Details connecting a computer to the network for remote monitoring.

Monitor the Instrument

Explains how to view status, send/download experiments, and configure notifications.

Maintain the System

Regular Maintenance

Outlines the recommended maintenance schedule for instruments and computers.

Infrequent Maintenance

Covers procedures for resolving issues and performing less common maintenance.

How to Troubleshoot the Installation

StepOne Software Problems

Provides steps to uninstall and reinstall StepOne software if errors occur.

Network Connection Problems

Offers a workflow to resolve issues with instrument remote monitoring.

Background Calibration Failure

Guides on troubleshooting failed background calibration runs.

Dye Calibration Failure

Guides on troubleshooting failed dye calibration runs and analyzing spectra.

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