Applied Biosystems StepOne User Manual

DRAFT September 25, 2007 1:07 am, 4376782_Colocated_Installation.fm
Chapter 3 Install the Colocated Layout
Perform the RNase P Experiment
Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
40
Notes
Set Up the Experiment
Prepare the TaqMan
®
RNase P Fast Instrument Verification Plate for the run.
Prepare the
RNase P Plate
IMPORTANT! Do not use an RNase P plate for another Applied Biosystems instrument
to verify the performance of the StepOne
™
system. RNase P plates for other instruments
contain the TAMRA
™
dye, which is not supported by the StepOne
™
system.
IMPORTANT! Wear powder-free gloves when you handle the RNase P plate .
1. Get the TaqMan
®
RNase P Fast Instrument Verification Plate from the freezer, then
allow the reaction plate to warm to room temperature (for approximately 5 min).
2. Remove the
RNase P plate from its packaging.
3. Vortex the reaction plate for 5 sec.
4. Centrifuge the reaction plate for 2 min at less than1500 rpm.
IMPORTANT! The reaction plate must be well mixed and centrifuged.
5. Confirm that the liquid is at the bottom of each well of the reaction plate. If not,
centrifuge the reaction plate again at a greater rpm and for a longer time.
IMPORTANT! Do not allow the bottom of the RNase P plate to become dirty. Fluids
and other contaminants that adhere to the bottom of the reaction plate can
contaminate the sample block(s) and cause an abnormally high background signal.
Correct Incorrect
Liquid is at the
bottom of the well.
• Not centrifuged with enough force, or
• Not centrifuged for enough time

Applied Biosystems StepOne Specifications

General

Brand	Applied Biosystems
Model	StepOne
Category	Laboratory Equipment
Language	English