Applied Biosystems StepOne User Manual

DRAFT September 25, 2007 1:07 am, 4376782_Standalone_Installation.fm
Chapter 4 Install the Standalone Layout
Perform the RNase P Experiment
Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
62
Notes
Set Up the Experiment
Prepare the TaqMan
®
RNase P Fast Instrument Verification Plate for the run.
Prepare the
RNase P Plate
IMPORTANT! Do not use an RNase P plate for another Applied Biosystems instrument
to verify the performance of the StepOne
™
system. RNase P plates for other instruments
contain the TAMRA
™
dye, which is not supported by the StepOne
™
system.
IMPORTANT! Wear powder-free gloves when you handle the RNase P plate .
1. Get the TaqMan
®
RNase P Fast Instrument Verification Plate from the freezer, then
allow the reaction plate to warm to room temperature (for approximately 5 min).
2. Remove the
RNase P plate from its packaging.
3. Vortex the RNase P plate for 5 sec.
4. Centrifuge the RNase P plate for 2 min at less than1500 rpm.
IMPORTANT! The RNase P plate must be well mixed and centrifuged.
5. Confirm that the liquid is at the bottom of each well of the RNase P plate. If not,
centrifuge the plate again at a greater rpm and for a longer time.
IMPORTANT! Do not allow the bottom of the RNase P plate to become dirty. Fluids
and other contaminants that adhere to the bottom of the RNase P plate can
contaminate the sample block(s) and cause an abnormally high background signal.
Correct Incorrect
Liquid is at the
bottom of the well.
• Not centrifuged with enough force, or
• Not centrifuged for enough time

Applied Biosystems StepOne Specifications

General

Brand	Applied Biosystems
Model	StepOne
Category	Laboratory Equipment
Language	English