Applied Biosystems StepOne User Manual

DRAFT September 25, 2007 1:07 am, 4376782_Troubleshoot.fm
Appendix A How to Troubleshoot the Installation
Dye Calibration Failure
Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
134
Notes
Dye Calibration Failure
Use this topic to troubleshoot failed dye calibrations.
Troubleshooting
Workflow
Start
Does the instrument
complete the dye
calibration?
No
Ye s
No
Ye s
Does the
calibration pass?
Do the dye spectra
peak in the correct
filters?
No
Reattempt the calibration. If the
problem persists, contact
Applied Biosystems as explained in
“How to Obtain Support” on page x.
One or more wells
produced spectra:
• Beyond the detectable
range for the system, or
• In more than one filters
Review the spectra for
irregularities as explained in
“Analyze the Calibration Data”
on page 106.
If the peaks of one or more spectra appear in the wrong
filter, confirm that:
• The spectral calibration plate was run in the correct
orientation (the
plate was not rotated 180° when run).
• The correct dyes were loaded onto the spectral
calibration plate, if you are running a custom plate.

Applied Biosystems StepOne Specifications

General

Brand	Applied Biosystems
Model	StepOne
Category	Laboratory Equipment
Language	English