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Applied Biosystems StepOne User Manual

Applied Biosystems StepOne
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DRAFT
September 25, 2007 1:07 am, 4376782_Get_Started.fm
Chapter 1 Get Started
About the StepOne
and StepOnePlus
Systems
Applied Biosystems StepOne
and StepOnePlus
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
CONFIDENTIAL — For AB Internal Use Only. Do Not Distribute.
2
Notes
About the StepOne
and StepOnePlus
Systems
There are two models available for this Real-Time PCR System:
The Applied Biosystems StepOne
and StepOnePlus
Real-Time PCR Systems use
fluorescent-based polymerase chain reaction (PCR) reagents to provide:
Quantitative detection of target nucleic acid sequences (targets) using real-time
analysis.
Qualitative detection of targets using post-PCR (endpoint) analysis.
Qualitative analysis of the PCR product (achieved by melt curve analysis that occurs
post-PCR).
System Features
Applied Biosystems StepOne
Real-Time
PCR System (StepOne
system)
48-well platform
Three-color system
Applied Biosystems StepOnePlus
Real-
Time PCR System (StepOnePlus
system)
96-well platform
Four-color system
•VeriFlex
sample blocks

Table of Contents

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Applied Biosystems StepOne Specifications

General IconGeneral
BrandApplied Biosystems
ModelStepOne
CategoryLaboratory Equipment
LanguageEnglish

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