Applied Biosystems StepOne - About the StepOne and StepOnePlus Systems

DRAFT September 25, 2007 1:07 am, 4376782_Get_Started.fm
Chapter 1 Get Started
About the StepOne
™
and StepOnePlus
™
Systems
Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
CONFIDENTIAL — For AB Internal Use Only. Do Not Distribute.
2
Notes
About the StepOne
™
and StepOnePlus
™
Systems
There are two models available for this Real-Time PCR System:
The Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems use
fluorescent-based polymerase chain reaction (PCR) reagents to provide:
• Quantitative detection of target nucleic acid sequences (targets) using real-time
analysis.
• Qualitative detection of targets using post-PCR (endpoint) analysis.
• Qualitative analysis of the PCR product (achieved by melt curve analysis that occurs
post-PCR).
System Features
Applied Biosystems StepOne
™
Real-Time
PCR System (StepOne
™
system)
• 48-well platform
• Three-color system
Applied Biosystems StepOnePlus
™
Real-
Time PCR System (StepOnePlus
™
system)
• 96-well platform
• Four-color system
•VeriFlex
™
sample blocks