Applied Biosystems StepOne User Manual

DRAFT September 25, 2007 1:07 am, 4376782_Maintain_the_System.fm
Chapter 6 Maintain the System
Regular Maintenance
Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
88
Notes
Regular Maintenance
Maintenance
Schedule
Perform routine maintenance of your Applied Biosystems StepOne
™
or StepOnePlus
™
Real-Time PCR Instrument and computer to ensure proper operation. The following table
displays the recommended maintenance schedule.
Connection
Required for
Maintenance
The spatial, background, and dye calibrations must be performed while the system is in a
colocated layout. To set up the system for maintenance, connect the yellow StepOne
system cable between the:
• Yellow Ethernet port ( ) of the instrument, and
• Ethernet port ( ) of the computer running the StepOne
™
software
IMPORTANT! Do not connect the StepOne system cable to the blue LAN Port ( ),
which is reserved for a network connection.
Maintenance
Notifications
When a required maintenance task such as a calibration is overdue, the StepOne
™
software and instrument display messages that prompt you to perform the necessary
procedure.
Perform
every...
Tas k
See
Page
Week Check the computer disk space. If necessary, archive or back up your
experiment files.
113
Power off the computer controlling the instrument, then after 30 sec
power on the computer.
—
Clean the surface of the instrument with a lint-free cloth. —
Defragment the computer hard drive. —
Month Perform a background calibration.
‡
‡ You can run a background calibration to check for contamination. Also, you must run a
background calibration and a dye calibration if any part of the instrument optics are replaced or
moved.
94
1.5 Years Perform a spatial calibration. 90
Perform a dye calibration. 100
Ethernet

Applied Biosystems StepOne Specifications

General

Brand	Applied Biosystems
Model	StepOne
Category	Laboratory Equipment
Language	English