Applied Biosystems StepOne - Install the Standalone Layout

Chapter 4
49
Applied Biosystems StepOne
™
and StepOnePlus
™
Real-Time PCR Systems
Installation, Networking, and Maintenance Guide
Notes
DRAFT September 25, 2007 1:07 am, 4376782_Standalone_Installation.fm
Install the Standalone Layout
This chapter covers:
■ About the Standalone Installation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
■ Set Up the Computer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Install the Computer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
Install the StepOne
™
Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
Prepare the StepOne
™
Software for Standalone Use . . . . . . . . . . . . . . . . . . . . . . 58
■ Perform the RNase P Experiment. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
Set Up the Experiment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Run the Experiment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
■ Analyze the RNase P Experiment. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
Unload the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
Transfer the Experiment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Analyze the Experiment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
■ After the Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
Note: For more information about any of the topics discussed in this guide, access the
Help from within the Applied Biosystems StepOne
™
Real-Time PCR System Software
by pressing F1, or clicking in the toolbar, or selecting HelpStepOne Software
Help.