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Applied Biosystems 7900HT - User Manual

Applied Biosystems 7900HT
384 pages
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Applied Biosystems
7900HT Fast Real-Time PCR System
and SDS Enterprise Database
User Guide

Table of Contents

Other manuals for Applied Biosystems 7900HT

Questions and Answers

  • M
    Melissa RiceJul 31, 2025
    What to do if the Applied Biosystems 7900HT Laboratory Equipment software crashes or freezes?
    • L
      Lauren JosephJul 31, 2025
      If the software crashes, freezes your computer, or displays an error message with your Applied Biosystems Laboratory Equipment, update the software to the latest version.
  • A
    Andre GloverAug 4, 2025
    How to fix frozen installation of Applied Biosystems Laboratory Equipment?
    • R
      Ryan WashingtonAug 4, 2025
      If you are unable to finish the installation of your Applied Biosystems Laboratory Equipment because the install program appears to be frozen, restart the installation process.
  • S
    samantha31Aug 8, 2025
    What to do if I get a communication error on my Applied Biosystems Laboratory Equipment?
    • J
      John BarnesAug 8, 2025
      If you encounter a communication error with your Applied Biosystems Laboratory Equipment, verify the cable connections and ensure that all devices are properly powered on.
  • G
    Gary MorrisonAug 13, 2025
    How to fix Plate Handler arm releases plates awkwardly into the plate stack in Applied Biosystems Laboratory Equipment?
    • M
      Melissa JordanAug 13, 2025
      If the Plate Handler arm releases plates awkwardly into the plate stack of your Applied Biosystems Laboratory Equipment, you should calibrate the release mechanism of the Plate Handler.
  • A
    Andrew CamposAug 16, 2025
    How to adjust Plate Handler arm contacts racks when retrieving or stacking plates in Applied Biosystems Laboratory Equipment?
    • H
      Hannah RitterAug 17, 2025
      If the Plate Handler arm contacts racks when retrieving or stacking plates in your Applied Biosystems Laboratory Equipment, adjust the alignment of the Plate Handler.
  • A
    Andrew ManningAug 21, 2025
    What to do if Plate Handler fails to sense or grasp plates in Applied Biosystems 7900HT Laboratory Equipment?
    • J
      Joshua MartinAug 21, 2025
      If the Plate Handler fails to sense or grasp plates in your Applied Biosystems Laboratory Equipment, clean the sensors and gripper fingers.
  • W
    Wendy MatthewsAug 26, 2025
    Why does my Applied Biosystems 7900HT Laboratory Equipment Plate Handler emit a grinding noise?
    • J
      John HammondAug 26, 2025
      If the Plate Handler emits a grinding noise when picking up or putting down plates in your Applied Biosystems Laboratory Equipment, inspect the Plate Handler for obstructions.

Summary

Safety and EMC Compliance Information

General Instrument Safety

Covers safe practices for moving, lifting, and operating the instrument to prevent injury.

Chemical Safety

Provides guidelines for safe handling, storage, and disposal of chemicals used with the instrument.

Electrical Safety

Addresses hazards related to high voltage, fuses, and proper grounding to prevent electrical shock.

Laser Safety

Explains Class 3B laser hazards and safety precautions for the instrument's laser system.

Chapter 1 Product Overview

Section 1.1 Getting to Know the Hardware

Introduces the hardware components of the 7900HT Fast Real-Time PCR System.

Section 1.2 Getting to Know the Software

Describes the software components used with the 7900HT instrument.

Chapter 2 Getting Started

Powering On the 7900 HT Instrument

Provides step-by-step instructions for powering on the instrument and its components.

Using the SDS Software

Guides users on how to start and navigate the SDS software interface.

Chapter 3 Preparing a Run

Step 1 - Creating a Plate Document

Guides users through the process of creating a plate document in the SDS software.

Step 9 - Running the Plate on the 7900 HT Instrument

Provides instructions on how to run prepared plates on the 7900HT instrument.

Chapter 4 Operating the Instrument

Section 4.1 Consumable Preparation

Covers preparation of consumables like optical plates and TaqMan Low Density Arrays.

Section 4.2 Running an Individual Plate

Details the process of running a single plate on the 7900HT instrument.

Section 4.3 Automated Operation

Explains how to operate the instrument using the Automation Controller Software.

Chapter 5 Analyzing End-Point Data

Section 5.1 Allelic Discrimination

Covers the process of analyzing allelic discrimination data.

Analyzing an Allelic Discrimination Run

Details the process of analyzing allelic discrimination data using the SDS software.

Chapter 6 Analyzing Real-Time Data

Chapter 7 Maintaining the Instrument

Chapter 8 Troubleshooting

Troubleshooting Table

Provides a table to help diagnose and resolve problems encountered with the system.

Appendix B Designing TaqMan Reagent-Based Assays

Appendix C Kits, Reagents, and Consumables

Applied Biosystems 7900HT Specifications

General IconGeneral
TypeReal-Time PCR System
Detection MethodFluorescence
Number of Channels5
SoftwareSDS Software
Reaction SpeedFast
Data AcquisitionReal-time
Sample Capacity384-well plate
Temperature Range4°C to 100°C
ApplicationQuantitative PCR
Format96-well or 384-well plates
Sample ThroughputHigh-throughput
Instrument TypeThermal Cycler
Optical SystemLED-based
Power Requirements100-240 VAC
Excitation SourceLED
Excitation WavelengthsMultiple wavelengths (depending on dyes used)
Emission WavelengthsMultiple wavelengths (depending on dyes used)

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