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Applied Biosystems 7900HT User Manual

Applied Biosystems 7900HT
384 pages
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Applied Biosystems
7900HT Fast Real-Time PCR System
and SDS Enterprise Database
User Guide

Table of Contents

Other manuals for Applied Biosystems 7900HT

Questions and Answers:

Applied Biosystems 7900HT Specifications

General IconGeneral
TypeReal-Time PCR System
Detection MethodFluorescence
Number of Channels5
SoftwareSDS Software
Reaction SpeedFast
Data AcquisitionReal-time
Sample Capacity384-well plate
Temperature Range4°C to 100°C
ApplicationQuantitative PCR
Format96-well or 384-well plates
Sample ThroughputHigh-throughput
Instrument TypeThermal Cycler
Optical SystemLED-based
Power Requirements100-240 VAC
Excitation SourceLED
Excitation WavelengthsMultiple wavelengths (depending on dyes used)
Emission WavelengthsMultiple wavelengths (depending on dyes used)

Summary

Safety and EMC Compliance Information

General Instrument Safety

Covers safe practices for moving, lifting, and operating the instrument to prevent injury.

Chemical Safety

Provides guidelines for safe handling, storage, and disposal of chemicals used with the instrument.

Electrical Safety

Addresses hazards related to high voltage, fuses, and proper grounding to prevent electrical shock.

Laser Safety

Explains Class 3B laser hazards and safety precautions for the instrument's laser system.

Chapter 1 Product Overview

Section 1.1 Getting to Know the Hardware

Introduces the hardware components of the 7900HT Fast Real-Time PCR System.

Section 1.2 Getting to Know the Software

Describes the software components used with the 7900HT instrument.

Chapter 2 Getting Started

Powering On the 7900HT Instrument

Provides step-by-step instructions for powering on the instrument and its components.

Using the SDS Software

Guides users on how to start and navigate the SDS software interface.

Chapter 3 Preparing a Run

Step 1 – Creating a Plate Document

Guides users through the process of creating a plate document in the SDS software.

Step 9 – Running the Plate on the 7900HT Instrument

Provides instructions on how to run prepared plates on the 7900HT instrument.

Chapter 4 Operating the Instrument

Section 4.1 Consumable Preparation

Covers preparation of consumables like optical plates and TaqMan Low Density Arrays.

Section 4.2 Running an Individual Plate

Details the process of running a single plate on the 7900HT instrument.

Section 4.3 Automated Operation

Explains how to operate the instrument using the Automation Controller Software.

Chapter 5 Analyzing End-Point Data

Section 5.1 Allelic Discrimination

Covers the process of analyzing allelic discrimination data.

Analyzing an Allelic Discrimination Run

Details the process of analyzing allelic discrimination data using the SDS software.

Chapter 6 Analyzing Real-Time Data

Chapter 7 Maintaining the Instrument

Chapter 8 Troubleshooting

Troubleshooting Table

Provides a table to help diagnose and resolve problems encountered with the system.

Appendix B Designing TaqMan Reagent-Based Assays

Appendix C Kits, Reagents, and Consumables

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