Applied Biosystems 7900HT User Manual

DRAFT
September 1, 2004 11:39 am, CH_Maintenance.fm
Chapter 7 Maintaining the Instrument
7-6 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
Replacing the Sample Block
When to Perform
IMPORTANT! Before changing the sample block, perform all required upgrades to
the SDS software and instrument firmware. Failure to update the software can make
the instrument inoperable or result in damage to instrument components.
You need to remove the 7900HT instrument sample block when you:
• Decontaminate the wells of the sample block (see page 7-14)
• Change sample block formats
IMPORTANT! Always run a background plate after installing the sample block.
Sample Block
Installation
Workflow
Unless instructed to do otherwise, adhere to the following guidelines when
exchanging sample block modules of different formats.
To install a sample block module:
1. Perform all required software and firmware upgrades to the Applied Biosystems
7900HT Fast Real-Time PCR System (see page 7-55).
2. Remove the existing sample block (see page 7-7).
3. Install the new sample block (see page 7-10).
4. Change the plate adapter (see page 7-12).
5. Run a background plate to check the sample block for contamination
(see page 7-16).
If you are changing block formats or installing a new block, also do the following:
IMPORTANT!
Perform pure dye and background runs after replacing the sample
block, even if you are replacing a sample block module with another block of the
same format.
6. Run a pure dye plate or microfluidic pure dye card to create the pure spectra
calibration values for the new format (see page 7-20).
7. Run the appropriate TaqMan
®
RNase P Instrument Verification Plate or a
TaqMan
®
Low Density 7900HT Installation Array (TGF-β card) to confirm the
proper operation of the sample block (see page 7-30).
If you are using an Automation Accessory, also do the following:
8. If changing consumable formats (for example, when replacing a Standard
384-Well Block with a Standard 96-Well Block, a Fast 96-Well Block, or a
7900HT System TaqMan
®
Low Density Array Upgrade), adjust the plate sensor
switch on the Plate Handler arm for the new plates (see page 7-37).
9. Align the Zymark
®
Twister Microplate Handler fixed-position bar code reader
for the new plate format (see page 7-41).
IMPORTANT! You must align the Plate Handler to only the Instrument position
(Zymark position 2) as explained on pages 7-41 to 7-44.
10. Align the fixed-position bar code reader for the new plate format
(see page 7-49).

Applied Biosystems 7900HT Specifications

General

Brand	Applied Biosystems
Model	7900HT
Category	Laboratory Equipment
Language	English