Applied Biosystems 7900HT - Chapter 5 Analyzing End-Point Data; End-Point Runs on the 7900 HT Instrument

DRAFT
September 1, 2004 11:39 am, CH_End-Point.fm
Chapter 5 Analyzing End-Point Data
5-2 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
End-Point Runs on the 7900HT Instrument
End-Point Runs
End-point is the term used to describe the category of sequence detection runs in
which the Applied Biosystems 7900HT Fast Real-Time PCR System is used to
measure the fluorescence of a biological sample after it has undergone thermal
cycling. Unlike real-time runs that can yield quantitative measurements, the focus of
end-point experiments is typically to produce a qualitative result. End-point analysis
is commonly used in combination with TaqMan
®
reagent-based chemistry to confirm
the presence or absence of specific target nucleic acid sequence in cells, tissues, or
fluid samples.
Currently, the SDS software supports one type of end-point analysis: Allelic
Discrimination.