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Applied Biosystems 7900HT - Section 6.3 Dissociation Curve Analysis

Applied Biosystems 7900HT
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DRAFT
September 1, 2004 11:39 am, CH_Real-Time.fm
Section 6.3 Dissociation Curve Analysis
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 6-37
Section 6.3 Dissociation Curve Analysis
In This Section
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-38
Before You Begin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-39
Analysis Checklist. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-40
Opening the Run Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-41
Analyzing the Run Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-41
Determining T
m
Values for the Analyzed Run . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-42
After the Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-44

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