DRAFT
September 1, 2004 11:39 am, CH_Run.fm
Chapter 3 Preparing a Run
3-4 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
Before You Begin
Background
Information
Chapter 5, “Analyzing End-Point Data,” and Chapter 6, “Analyzing Real-Time Data,”
include brief discussions of the experiments that you can perform using the 7900HT
instrument. Before beginning, you may want to review the appropriate chapter for
your experiment:
Allelic Discrimination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-5
Absolute Quantification. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-5
Relative Quantification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-15
Dissociation Curve Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-37
Getting More
Information from
the SDS
Online Help
The Sequence Detection Systems Software Online Help can guide you through the
procedures for setting up, performing, and analyzing runs. To get help at any time,
click the button located inside the dialog box or window in which you are
working.
Maximizing
Throughput for
End-Point Runs
For end-point applications such as allelic discrimination, the throughput of the
Applied Biosystems 7900HT Fast Real-Time PCR System can be increased by
dividing the workload between the 7900HT instrument and several thermal cyclers.
Unlike real-time runs, the 7900HT instrument collects data for end-point runs after
the completion of the PCR. Consequently, you can perform the thermal cycling of
end-point plates elsewhere and then transfer them to the 7900HT instrument
afterwards for data collection and analysis.
IMPORTANT! To perform the thermal cycling and the plate read using the 7900HT
instrument, run the plate first as a real-time plate document and then again as an
allelic discrimination plate document (see “Step 5 – Programming the Plate
Document Method” on page 3-19 for the procedure).
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