DRAFT
September 1, 2004 11:39 am, CH_Real-Time.fm
Analysis Checklist
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 6-9
Analysis Checklist
Workflow
Overview
1. Open the plate document (see page 6-10).
2. Configure the analysis settings for the run (see page 6-11).
3. Analyze the run data (see page 6-12).
4. For each detector that you configured to determine the baseline and threshold
values (see page 6-46):
– Automatically – Verify that the baseline and threshold values for the detector
were set correctly by the software.
– Manually – Set the baseline and threshold values for the detector
5. Visualize outliers and eliminate any outlying amplification from the run data
(see page 6-49).
6. View the results of the absolute quantification run (see page 6-14).
7. Choose from the following post-analysis options (see page 6-52):
– Reanalyze the run data.
– Adjust the display settings for the results table, plate grid, and plate
document plots.
– Print elements of the plate document.
– Export the plate document results table or plots.
– Save the analysis.
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