Applied Biosystems 7900HT User Manual

DRAFT
September 1, 2004 11:39 am, CH_Maintenance.fm
Chapter 7 Maintaining the Instrument
7-22 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
Preparing the Pure Dye Plates or Cards
Preparing the
Pure Dye Plates
IMPORTANT! A background run must be performed before running a pure dye plate.
See “Performing a Background Run” on page 7-16 for more information.
1. Remove the pure dye plate from the refrigerator or freezer and set it aside to
warm to room temperature.
2. Remove the pure dye plate from its packaging.
3. Briefly centrifuge the pure dye plate.
Preparing the
Microfluidic Pure
Dye Cards
IMPORTANT! A background run must be performed before running the microfluidic
pure dye cards. See “Performing a Background Run” on page 7-16 for more
information.
1. Remove the three empty microfluidic pure dye cards from the refrigerator and
set them aside to warm to room temperature.
2. Remove the three dye tubes (FAM, VIC, and ROX dyes) from the freezer to
thaw. When the dyes have thawed, vortex the tubes.
3. Load the FAM dye into one of the empty microfluidic pure dye cards, loading
100 µL of dye per fill reservoir.
4. Centrifuge the microfluidic pure dye card per the procedures in “Centrifuging
the TaqMan Low Density Arrays” on page 4-16.
5. Seal the microfluidic pure dye card per the procedures in “Sealing the TaqMan
Low Density Arrays” on page 4-19.
6. Repeat steps 3 through 5 for the VIC and ROX dyes.
Preparing a Plate Document for a Pure Dye Plate or Card
Preparing a
Pure Dye Plate
Document
1. Start the SDS software.
2. Click (or select File > New).
3. Configure the New Document dialog box:
Assay – Select Pure Spectra.
Container – Select the appropriate format.
Template – If performing a:
• Standard 384-Well Pure Dye Run: select 384 Well Pure Dyes Plate.sdt.
If no plate document templates are available, construct a Pure Dye plate
document using the product insert from the Spectral Calibration Kit and
the procedure on page 7-28.
• Standard 96-Well Pure Dye Run: select the plate document template
matching the Pure Dye plate you intend to run.
To run Plate 1 (containing FAM, JOE
™
, NED, and ROX dyes), select
96 Well Pure Dyes Plate 1.sdt.
To run Plate 2 (containing SYBR, TAMRA, TET, and VIC dyes), select
96 Well Pure Dyes Plate 2.sdt.

Applied Biosystems 7900HT Specifications

General

Brand	Applied Biosystems
Model	7900HT
Category	Laboratory Equipment
Language	English