DRAFT
September 1, 2004 11:39 am, CH_Maintenance.fm
Performing a Pure Dye Run
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 7-23
• Fast 96-Well Pure Dye Run: select the plate document template matching
the Pure Dye plate you intend to run.
To run Plate 1 (containing FAM, JOE, NED, and ROX dyes), select
Fast 96 Well Pure Dyes Plate 1.sdt.
To run Plate 2 (containing SYBR, TAMRA, TET, and VIC dyes), select
Fast 96 Well Pure Dyes Plate 2.sdt.
• Microfluidic Pure Dye Run: leave the field blank.
To run the microfluidic pure dye card containing FAM dye,
select MFC FAM Pure Dyes.sdt.
To run the microfluidic pure dye card containing ROX dye,
select MFC ROX Pure Dyes.sdt.
To run the microfluidic pure dye card containing VIC dye,
select MFC VIC Pure Dyes.sdt.
4. In the Barcode field, scan the bar code number using the hand-held bar code
reader.
5. Click . The software displays a plate document with the attributes for a
pure dye run.
6. Save the Pure Dye plate document:
a. Click (or select File > Save).
b. Select Files of type > SDS 7900HT Document (*.sds).
c. In the File name field, if running a:
• Standard 384-Well Plate, enter PureDye_<date in MMDDYY format>
For example, the file name for a plate run on May 31, 2001 would be:
PureDye_053101.
• Standard 96-Well Plate, enter PureDye_Plate<plate #>_<date in
MMDDYY format>
For example, the file name for a Pure Dye Plate 1 run on May 31, 2001
would be: PureDye_Plate1_053101.
• Fast 96-Well Plate, enter FastPureDye_Plate<plate #>_<date in
MMDDYY format>.sds
For example, for a fast pure dye plate 1 run on May 31, 2001, type:
FastPureDye_Plate1_053101.sds
• Microfluidic pure dye card, enter <Dye><date in MMDDYY format>_MFC
For example, the file name for a run performed on May 31, 2001 would be:
FAM_053101_MFC.
d. Click .
The software saves the plate document.
7. Prepare and run the pure dye plate or card as explained below.
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