DRAFT
September 1, 2004 11:38 am, CH_Overview.fm
Chapter 1 Product Overview
1-4 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
7900HT Instrument
Internal
Components
The 7900HT instrument contains the hardware used for thermal cycling and
detection of fluorescent chemistries (see page 1-2). Figure 1-3 illustrates the major
subcomponents of the instrument described in detail below.
Do not remove the cover to the Applied Biosystems 7900HT Fast
Real-Time PCR System. Only a qualified Applied Biosystems service engineer may
repair or adjust the internal components of the 7900HT instrument. Failure to comply
can result in serious injury and/or damage to the instrument.
Figure 1-3 Internal Components of the 7900HT Instrument
Automated Plate
Handling System
PHYSICAL HAZARD. Keep hands and loose clothing away
from the instrument tray and door at all times during instrument operation. The
7900HT instrument contains several internal components that can cause serious
physical injury.
The 7900HT instrument features an automated plate-handling system to provide easy
loading and removal of plates from the instrument. In combination with the
automation module, the plate-handling system allows unattended operation of the
instrument.
Heated Clamp
PHYSICAL INJURY HAZARD. Hot Surface. Use care when
working around this area to avoid being burned by hot components.
The 7900HT instrument has a heated clamp that provides optimal heat transfer and
uniform heating during thermal cycling. When the instrument tray loads a plate, the
clamp applies a downward pressure of 70 lbs (31.8 kg) onto the consumable. During
the run, the clamp maintains a constant temperature of 105 °C (±3 °C) to prevent
condensation within the consumable wells.
GR2112
CCD
camera
Reader
Heated clamp
Tray
Laser
Door
Optics
system
Automated plate
handling system
Sample block module
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