Applied Biosystems 7900HT - Detectors List

DRAFT
September 1, 2004 11:39 am, App_SWReference.fm
Setup Table Files
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide A-7
6. Detectors List
Description
The detector list consists of one or more lines displaying the information for each
detector used on the plate document.
• The Detectors List section must contain one line (or definition) for each
detector present on the plate.
• The number of lines in the Detectors List section must be equal to the number
defined in the Number of Detectors section (see number 4 above).
• Leave blank the Quencher Dye entry for detectors created for the SYBR Green I
Dye or probes labeled with a non-fluorescent quencher.
Format (for a
single detector):
[ detector name <tab> reporter dye <tab> quencher dye <tab>
description <tab> comments <cr> ]
Examples:
Example Code A-2 Allelic discrimination setup table file
*** SDS Setup File Version 3
*** Output Plate Size 384
*** Output Plate ID 384N75822034
*** Number of Detectors 2
Detector Reporter Quencher Description Comments
CYP 2C9*2.1 FAM PDAR CYP 2C9*2 Al1 Example Probe
CYP 2C9*2.2 VIC PDAR CYP 2C9*2 Al2 Example Probe
...
Example Code A-3 Absolute or relative quantification setup table file
*** SDS Setup File Version 3
*** Output Plate Size 96
*** Output Plate ID 96JG729FF903
*** Number of Detectors 3
Detector Reporter Quencher Description Comments
GAPDH VIC GAPDH Probe Example Probe
SYBR Green SYBR SYBR Green I Example Probe
RNase P FAM TAMRA RNase P Probe Example Probe
...