Applied Biosystems 7900HT User Manual

DRAFT
September 1, 2004 11:38 am, CH_GetStart.fm
Chapter 2 Getting Started
2-2 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
Getting Started
Before You Begin
If this is your first time using the Applied Biosystems 7900HT Fast Real-Time PCR
System, consider completing the “Basic Software Skills Tutorial” on page 2-10
before continuing. The tutorial will provide you with the fundamental knowledge
required to operate the SDS software and will teach you timesaving techniques to
allow you to use it quickly and efficiently.
Procedure
Quick Reference
The following table contains a list of major procedures described inside this manual.
Setting Up and Running SDS Experiments
Creating an SDS Plate Document . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-9
Running an Individual SDS Plate Document . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-23
Running Batches of SDS Plate Document . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-31
Stopping a Run from the SDS Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-28
Stopping a Run from the Automation Controller Software . . . . . . . . . . . . . . . . .4-41
Ejecting a Plate from the SDS Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-29
Ejecting a Plate from the Automation Controller Software . . . . . . . . . . . . . . . . .4-41
Analyzing Run Data
Analyzing an Allelic Discrimination Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-5
Analyzing an Absolute Quantification Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-5
Analyzing an Relative Quantification Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-15
Analyzing a Dissociation Curve (Melting Curve) Run . . . . . . . . . . . . . . . . . . . . .6-37
Maintaining the 7900HT Instrument
Changing the Plate Adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-12
Replacing the Sample Block . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-6
Decontaminating the Sample Block . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-14
Performing a Background Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-16
Performing a Pure Dye Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-20
Adding Custom Dyes to the Pure Dye Set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-27
Verifying Instrument Performance Using a TaqMan
®
RNase P Instrument
Verification Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-30
Maintaining the Automation Accessory
Adjusting the Sensitivity of the Plate Sensor Switch . . . . . . . . . . . . . . . . . . . . . .7-37
Aligning the Plate Handler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-41
Aligning the Fixed-Position Bar Code Reader . . . . . . . . . . . . . . . . . . . . . . . . . . .7-49
Cleaning and Replacing Gripper Finger Pads . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-52
Note: The Automation Accessory includes the Zymark
®
Twister Microplate Handler
and the fixed-position bar code reader. See “Instrument Components” on page 1-3
for more information.

Applied Biosystems 7900HT Specifications

General

Brand	Applied Biosystems
Model	7900HT
Category	Laboratory Equipment
Language	English