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Applied Biosystems 7900HT - Chapter 8 Troubleshooting

Applied Biosystems 7900HT
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DRAFT
September 1, 2004 11:39 am, CH_Trouble.fm
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 8-1
8
Troubleshooting 8
In This Chapter
Troubleshooting Table . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-2
Low Precision or Irreproducibility . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-5
Background Runs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-9
Pure Dye Runs. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-11
Real-Time Runs (Quantitative PCR and Dissociation Curves). . . . . . . . . . . . . . . 8-12
End-Point Runs (Allelic Discrimination) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-13
Software and 7900HT Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-14
Zymark Twister Microplate Handler and Fixed-Position Bar Code Reader . . . . . 8-17
TaqMan Low Density Array . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-18
SDS Enterprise Database . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8-20

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