Applied Biosystems 7900HT User Manual

DRAFT
September 1, 2004 11:39 am, CH_Trouble.fm
Background Runs
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 8-9
Background Runs
Background
Troubleshooting
Tabl e
Isolating
Sample Block
Contamination
Signals exceeding 2500 FSU are considered outside the limit of normal background
fluorescence and indicates that the either the background plate or the sample block
module may be contaminated.
1. If not already open, open the plate document for the background run.
2. In the toolbar, click (Hide/Show System Raw Data Pane).
The SDS software displays the raw data pane for the background run.
3. Select all wells in the plate document.
4. Inspect the raw background data for an aberrant spectral peak or peaks.
Wells producing raw spectra that exceed 2500 FSU are considered irregular and
could be contaminated. The following figure illustrates the raw data produced
by a run on a sample block module containing a contaminated well.
Table 8-2 Troubleshooting Background Runs
Observation Possible Cause Recommended Action
Software will not extract
background data
During setup, the wrong
plate type was assigned to
the plate document
Run a new background
plate document with the
proper plate type setting.
Background is too high
(≥ 2500 FSU
a
)
aFluorescent standard units – The measure of amplitude displayed along the Y-axis of the
Background Plot.
See below.
Background is too high
(=>2500)
Sample block
contamination
1. Construct and run a new
background plate.
2. See “Isolating
Sample Block
Contamination” below.
Background plate
contamination
Possible contamination

Applied Biosystems 7900HT Specifications

General

Brand	Applied Biosystems
Model	7900HT
Category	Laboratory Equipment
Language	English