Applied Biosystems 7900HT - Page 298

DRAFT
September 1, 2004 11:39 am, CH_Trouble.fm
Chapter 8 Troubleshooting
8-10 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
5. Identify the location(s) of the contaminated well(s) on the sample block by
selecting increasingly smaller regions of the plate document (see below).
a. The raw data from
the selected wells
contains the peak.
The contaminated
well must be in
columns 7-12.
b. The raw data from
the selected wells
does not contain
the peak.
The contaminated
well must be in the
last four wells of
columns 7-12.
c. The raw data from
the selected wells
contains the peak.
The contaminated
well must be in the
last four wells of
columns 10-12.
d. The raw data from
the selected wells
contains the peak.
The contaminated
well must be in the
last two wells of
columns 10-12.
e. By selecting each
of the wells from
the last two wells
of columns 10-12,
the location of the
contaminated
well (G10) is
determined.