Applied Biosystems 7900HT - After the Analysis

DRAFT
September 1, 2004 11:39 am, CH_Real-Time.fm
Chapter 6 Analyzing Real-Time Data
6-14 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
Viewing the
Analysis Table
Displays the results of the absolute quantification calculation in the results table of
the plate document. Figure 6-6 shows an example of the results table containing the
data from an RNase P Instrument Verification Plate.
Figure 6-6 Analysis Table of the Absolute Quantification Plate Document
The columns of the table are:
• Position – The coordinate position of the well on the plate.
• Sample – The sample name applied to the well.
• Detector – The name of the detector assigned to the well.
• Task –The task (NTC, Standard, or Unknown) assigned to the well.
Note: See page 3-16 for information on applying tasks to the plate document.
• C
T
– The threshold cycle generated by the well during the PCR.
Note: The software displays ‘Undetermined’ in the C
T
column when the
associated sample fails to produce a noticeable rise in fluorescent signal during
the entire PCR (the fluorescent signal produced by the well never crosses the
threshold defined for the associated detector).
• Quantity – For wells containing:
– Unknown samples, this column reports the extrapolated quantity value of the
sample in the same units of measure as the standard.
– Standard samples, this column displays the quantity assigned to the well.
Note: See page 3-17 for information on applying quantities to standard wells of
the plate document.
The following two columns contain data only if a well is run as part of a replicate group.
• Qty Mean – The arithmetic mean for the quantity values of the replicate group
associated with the well.
• Qty stddev – The standard deviation of the quantity values of the replicate
group associated with the well.
• Status – Indicates the analysis status of the sample data.
After the Analysis
User Access
Requirement
If using the SDS Enterprise Database, you must belong to the Scientist or
Administrator User Group to save the analyze data of an absolute quantification run.
Post-Analysis
Options
Changing the Display Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-52
Printing a Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-52
Exporting Plate Document Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-52
Saving the Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6-53
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