DRAFT
September 1, 2004 11:39 am, CH_Real-Time.fm
Chapter 6 Analyzing Real-Time Data
6-26 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
Analyzing the Study
User Access
Requirement
There is no access requirement. All users can analyze absolute quantification data
that has been saved to the SDS Enterprise Database.
Configuring the
Analysis Settings
Before analyzing the data for your study, you must specify the analysis settings for
each detector that you want to analyze. The settings are detector specific and must be
set for each detector individually. The following sections briefly discuss the analysis
settings provided by the software in the Analysis Settings dialog box:
• About the Analysis Settings (see below)
• Specifying the Analysis Settings (see page 6-29)
Note: You can analyze the run without configuring the analysis settings. However,
the software analyzes the data using the baseline of cycles 3 to 15 and a default
threshold value of 0.2.
About the Analysis Settings
C
T
Analysis for
Selected Detector
Settings
The settings of this group box control the method that the SDS software uses to
calculate the C
T
s of the sample replicate groups associated with the selected detector
in the Detector drop-down list. As shown in Figure 6-9, the Ct Analysis settings
provide both automatic and manual methods for determining the baseline and
threshold values.
Figure 6-9 C
T
Analysis for Selected Detectors Settings
• Automatic Ct – Instructs the software to calculate the optimal baseline range
and threshold values for the selected detectors using the AutoC
T
algorithm
(see page 6-18).
IMPORTANT! If you choose to use the AutoC
T
algorithm, Applied Biosystems
recommends that you verify that the baseline and threshold values are set
correctly for each detector configured for AutoCalling (see page 6-46).
Automatic Ct
option button
Manual Ct
option button
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