Applied Biosystems 7900HT User Manual

DRAFT
September 1, 2004 11:39 am, CH_Real-Time.fm
Getting Started
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 6-21
Getting Started
Using the SDS
Software
Online Help
For specific instructions on any procedure described within this section, refer to the
Sequence Detection Systems Software Online Help. To get help at any time during the
procedure, click located within the dialog box or window in which you are
working.
Examples in This
Chapter
The screenshots that appear within this chapter were created using a series of
TaqMan
®
Cytokine Gene Expression Plates (PN 4304671), a research tool for
real-time, in vitro quantitative evaluation of human cytokine gene expression. The
plate detects the expression of 12 cytokine target sequences and an endogenous
control in complementary DNA (cDNA) samples. The TaqMan Cytokine Gene
Expression Plate I consists of a MicroAmp
®
Optical 96-Well Reaction Plate arranged
into 24 replicate groups, each containing TaqMan probes and primers for the assay of
one human cytokine mRNA and an endogenous control. Figure 6-8 illustrates the
assay configurations for the plate.
The study used to produce the screenshots for this section consisted of four plates of
a time-course experiment run to evaluate the expression of 24 target cytokine mRNA
over a 36-hour period.
Figure 6-8 Sample Configuration of the Example Plates
Note:
For more information on the TaqMan Cytokine Gene Expression Plate I, see
the TaqMan Cytokine Gene Expression Plate I Protocol (PN 4306744).
GR2108
36 hr
24 hr
12 hr
0 hr
IL-1α IL-1βIL-2
IL-3 IL-4 IL-5
IL-6 IL-7 IL-8
IL-15 IL-17IL-13
IL-18 G-CSF GM-CSF
TGFβ TNFα TNFΒ
IL-10 IL-12p35 IL-12p40
M-CSF IFNγ LTβ
GR2108GR2108GR2108GR2108
Plate 1
Plate 2
Plate 3
Plate 4

Applied Biosystems 7900HT Specifications

General

Brand	Applied Biosystems
Model	7900HT
Category	Laboratory Equipment
Language	English