Applied Biosystems 7900HT User Manual

DRAFT
September 1, 2004 11:39 am, CH_Real-Time.fm
Chapter 6 Analyzing Real-Time Data
6-8 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
Before You Begin
Using the SDS
Software
Online Help
For specific instructions on any procedure described within this section, refer to the
Sequence Detection Systems Software Online Help. To get help at any time during the
procedure, click located within the dialog box or window in which you are
working.
Examples in This
Chapter
The illustrations and screenshots that appear within this chapter were created for a
TaqMan
®
RNase P 384-Well Instrument Verification Plate (PN 4323306), an
experiment run during the installation of the 7900HT instrument to verify its
performance. The sealed plate is preloaded with the reagents necessary for the
detection and quantification of genomic copies of the human RNase P gene (a
single-copy gene encoding the RNA moiety of the RNase P enzyme). Each well
contains pre-loaded reaction mix (TaqMan
®
2✕ Universal PCR Master Mix, RNase
P primers, and FAM
™
dye-labeled probe) and template.
Figure 6-4 illustrates the arrangement of standards and samples on the RNase P
Instrument Verification Plate. As shown below, the RNase P Instrument Verification
Plate consists of 5 columns of template standards (1250, 2500, 5000, 10,000, and
20,000 copies) and two replicate populations (5000 and 10,000 copies).
Figure 6-4 Sample Configuration of the Example Plate
GR2107
Population 1
5000
Population 2
10000
STD 1250 STD 2500 STD 5000
STD 10000
NTC
STD 20000

Applied Biosystems 7900HT Specifications

General

Brand	Applied Biosystems
Model	7900HT
Category	Laboratory Equipment
Language	English