Applied Biosystems 7900HT User Manual

DRAFT
September 1, 2004 11:39 am, CH_Real-Time.fm
Opening the Run Data
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 6-41
Opening the Run Data
User Access
Requirement
There is no access requirement. All users can open dissociation curve data that has
been saved to the SDS Enterprise Database.
Open the
Un-analyzed
Plate Document
Opening a Plate Document File
1. Click (or select File > Open).
2. In the Look in field, navigate to and select the plate document file.
3. Click .
The SDS software displays the plate document file.
4. Configure the Analysis Settings for each marker as explained below.
Opening Un-analyzed Data from the SDS Enterprise Database
5. Click (or select File > Open Document from Database).
6. In the Plate Query dialog box, configure the Find Plates Matching These
Criteria table with parameters that correspond to the session of interest.
7. Click .
8. In the Search Results list, scroll to and select the session that you want to
analyze.
9. Click .
10. Click when finished.
Analyzing the Run Data
User Access
Requirement
There is no access requirement. All users can analyze dissociation curve data that has
been saved to the SDS Enterprise Database.
Analyzing the
Run
The run data from a temperature ramp can be analyzed immediately following the
completion of the run. For an explanation of how the software manipulates the raw
data, see “Algorithmic Manipulation of Raw Data” on page 6-7.
1. Select all wells in the plate grid.
The software outlines the selected wells with a black border.
2. Choose one of the following:
• Select Analysis > Analyze.
• Click .
The software analyzes the run data and displays the results in the Dissociation
Curve tab.
3. Determine the T
m
for the dissociation curves displayed within the Dissociation
Curve tab as explained on page 6-44.

Applied Biosystems 7900HT Specifications

General

Brand	Applied Biosystems
Model	7900HT
Category	Laboratory Equipment
Language	English