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Applied Biosystems 7900HT User Manual

Applied Biosystems 7900HT
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DRAFT
September 1, 2004 11:39 am, CH_Basic.fm
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 4-1
4
Operating the Instrument 4
In This Chapter
Notes for Database Users . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
Before You Begin . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4
Section 4.1 Consumable Preparation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5
Preventing Contamination. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-6
Compatible Consumables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-6
Preparing Optical Plates for Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-8
Preparing TaqMan Low Density Arrays for Use. . . . . . . . . . . . . . . . . . . . . . . . . . 4-10
Section 4.2 Running an Individual Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-23
Saving the Plate Document . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-24
Running a Single Plate (Using the SDS Software) . . . . . . . . . . . . . . . . . . . . . . . . 4-25
After the Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-29
Section 4.3 Automated Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-31
Operating the Software with an SDS Enterprise Database . . . . . . . . . . . . . . . . . . 4-32
Operating the Software without an SDS Enterprise Database . . . . . . . . . . . . . . . 4-34
Running Plates Using the Automation Controller Software . . . . . . . . . . . . . . . . . 4-41
After the Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-44

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Applied Biosystems 7900HT Specifications

General IconGeneral
BrandApplied Biosystems
Model7900HT
CategoryLaboratory Equipment
LanguageEnglish

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