DRAFT
September 1, 2004 11:39 am, App_SWReference.fm
Appendix A Software Reference
A-8 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
Marker Definitions
Element numbers 7 to 9 define the markers that appear in the well descriptions that
follow in a later section. The marker definition consists of three sections: the
declaration of the number of markers, the marker list header, and the marker list.
7. Number of Markers (Allelic Discrimination Only)
IMPORTANT! Number of Markers information is required only in setup table files
created for allelic discrimination runs. This section does not appear in setup table
files created for absolute or relative quantification runs.
Description
This line defines the total number of markers on the plate.
Format
[ *** Number of Markers <tab> number of markers <cr> ]
Example
*** Number of Markers 2
8. Markers List Header (Allelic Discrimination Only)
IMPORTANT! Marker List Header information is required only in setup table files
created for allelic discrimination runs. This section does not appear in setup table
files created for absolute or relative quantification runs.
Description
This line contains the column headings for the Marker Definitions section of the
setup table file that make the file easier to edit using a program such as Microsoft
Excel.
Format
[ Marker <tab> AlleleX <tab> AlleleY <tab> Description <tab> Comments
<cr> ]
Example
Example Code A-4
Marker AlleleX AlleleY Description Comments