Applied Biosystems 7900HT User Manual

DRAFT
September 1, 2004 11:39 am, CH_Maintenance.fm
Chapter 7 Maintaining the Instrument
7-14 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
Decontaminating the Sample Block
When to Perform
The following procedure describes how to decontaminate the wells of a sample block
module. The procedure eliminates residual PCR-related products, including
fluorescent labeled TaqMan
®
probes. Clean the sample block as often as needed.
IMPORTANT! If you plan to use a cleaning or decontamination method other than
the one in this manual, check with Applied Biosystems first to ensure that the
method will not damage the sample block module or the 7900HT instrument.
Materials
Required
• 10% Sodium hypochlorite (bleach) solution
• Isopropanol, 100 percent pure
• 5/32 in hex key
•Cotton swabs
CHEMICAL HAZARD. Sodium hypochlorite (bleach) is a
liquid disinfectant that can be corrosive to the skin and can cause skin
depigmentation. Please read the MSDS, and follow the handling instructions. Wear
appropriate protective eyewear, clothing, and gloves.
CHEMICAL HAZARD. Isopropanol is a flammable liquid and
vapor. It may cause eye, skin, and upper respiratory tract irritation. Prolonged or
repeated contact may dry skin and cause irritation. It may cause central nervous
system effects such as drowsiness, dizziness, and headache, etc. Please read the
MSDS, and follow the handling instructions. Wear appropriate protective eyewear,
clothing, and gloves.
Cleaning a Low
Density Array
Block
This procedure applies to the sample block on the 7900HT System TaqMan Low
Density Array Upgrade.
1. Wipe the surface of the sample block (grey aluminum) with 10% bleach.
2. Wipe the sample block three times with distilled water.
3. Wipe the sample block with isopropanol, then allow to air dry.
4. Replace the block as explained in “Replacing the Sample Block” on page 7-10.
5. Run a background Low Density Array to confirm that the contamination has
been removed.
Warning
GR2028
Well A-1
Circuitry and connections to
the instrument (Do Not Touch)

Applied Biosystems 7900HT Specifications

General

Brand	Applied Biosystems
Model	7900HT
Category	Laboratory Equipment
Language	English