DRAFT
September 1, 2004 11:39 am, CH_Real-Time.fm
Analyzing the Data
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide 6-11
Analyzing the Data
User Access
Requirement
There is no access requirement. All users can analyze absolute quantification data
that has been saved to the SDS Enterprise Database.
Configuring the
Analysis Settings
Before analyzing the plate document data, you must configure the analysis settings
for each detector that you want to analyze. The analysis settings are detector-specific
and must be set for each one individually. For each detector, the SDS software can
calculate baseline and threshold values automatically using the AutoC
T
algorithm, or
manually using values you supply.
Note: You can analyze the run without configuring the analysis settings, however the
software will analyze the data using a default baseline of cycle 3 to 15 and a default
threshold value of 0.2.
1. Click (or select Analysis menu > Analysis Settings).
2. In the Detector drop-down list, select a detector.
3. In the Analysis Settings dialog box, configure the method (automatic or manual)
that the software will use to determine the baseline and threshold values for the
selected detectors:
To determine the baseline and threshold values:
Automatically – Select the Automatic C
T
option button.
IMPORTANT! If you choose to use the AutoC
T
algorithm, verify that the
baseline and threshold were called correctly for each autocalled detector
following the analysis (use the procedure on page 6-46).
Note: See “Automatic Threshold and Baseline Determination” on page 6-18 for
more information on the AutoC
T
algorithm.
Manually – Do the following:
a. Select the Manual Ct option button.
b. In the Threshold field, enter a threshold value to apply to the selected
detector(s) or leave the field empty and set the threshold value manually as
explained on page 6-46.
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