Applied Biosystems 7900HT User Manual

DRAFT
September 1, 2004 11:39 am, CH_Basic.fm
Chapter 4 Operating the Instrument
4-34 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
Operating the Software without an SDS Enterprise Database
Overview
The first step in configuring the Applied Biosystems 7900HT Fast Real-Time PCR
System for automated operation is to add plate documents to the plate queue. The
plate queue is a list of plate document files that the Automation Controller Software
uses to identify and run associated plates during automated operation. By adding
plate documents to the queue, the plate documents automatically become available
for use with the Automation Accessory.
IMPORTANT! After you have added a plate document to the plate queue, the
software locks the file, preventing any changes from being made to it until you run or
remove the plate document from the queue.
The Applied Biosystems 7900HT Fast Real-Time PCR System has three options for
adding plate documents to the plate queue. Review the options listed below, then
choose the method that best suits your needs.
• Add a plate document to the plate queue from the SDS software. (page 4-35)
• Using the Template Batch utility, create batches of plate documents from a plate
document template and add them to the plate queue. (page 4-36)
• Add or remove individual or multiple plate documents to the plate queue using
the Automation Controller Software. (page 4-39)
Workflow
1. Add plate documents to the plate queue.
– Add a plate document to the plate queue using the SDS Software (see
page 4-35)
– Create and add plate documents to the plate queue using the Template
Batch Utility (see page 4-36)
2. Start and configure the Automation Controller Software and add or remove plate
documents from the plate queue as needed. (see page 4-39)
3. Load prepared optical plates and Low Density Arrays onto the stacks of the
Zymark Twister Microplate Handler. (see page 4-41)
4. Run the instrument. (see page 4-43)
5. Analyze the run data.
– Allelic Discrimination Analysis (see page 5-5)
– Absolute Quantification Analysis (see page 6-5)
– Relative Quantification Analysis (see page 6-15)
– Dissociation Curve Analysis (see page 6-37)

Applied Biosystems 7900HT Specifications

General

Brand	Applied Biosystems
Model	7900HT
Category	Laboratory Equipment
Language	English