DRAFT
September 1, 2004 11:39 am, App_SWReference.fm
Setup Table Files
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide A-9
9. Markers List (Allelic Discrimination Only)
IMPORTANT! Marker List information is required only in setup table files created
for allelic discrimination runs. This section does not appear in setup table files
created for absolute or relative quantification runs.
Description
The marker list consists of one or more lines displaying the information for each
marker used on the plate document.
• The Markers List section must contain one line (or definition) for each marker
present on the plate.
• The number of lines in the Markers List section must be equal to the number
defined in the Number of Markers section (see number 7 above).
• Leave the markers list line blank in setup files generated for relative and
absolute quantification. (Real-time plate documents do not use markers.)
Format (for a
single marker):
[ marker name <tab> alleleX <tab> alleleY <tab> description <tab>
comments <cr> ]
Examples:
Example Code A-5 Allelic discrimination setup table file
*** SDS Setup File Version 3
*** Output Plate Size 384
*** Output Plate ID 384N75822034
*** Number of Detectors 2
Detector Reporter Quencher Description Comments
CYP 2C9*2.1 FAM PDAR CYP 2C9*2 Al1 Example Probe
CYP 2C9*2.2 VIC PDAR CYP 2C9*2 Al2 Example Probe
*** Number of Markers 1
Marker AlleleX AlleleY Description Comments
CYP 2C9*2 CYP 2C9*2.2 CYP 2C9*2.2 PDAR CYP 2C9*2 Example marker
...
Example Code A-6 Absolute or relative quantification setup table file
*** SDS Setup File Version 3
*** Output Plate Size 96
*** Output Plate ID 96JG729FF903
*** Number of Detectors 3
Detector Reporter Quencher Description Comments
GAPDH VIC GAPDH Probe Example Probe
SYBR Green SYBR SYBR Green I Example Probe
RNase P FAM TAMRA RNase P Probe Example Probe
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