DRAFT
September 1, 2004 11:39 am, App_SWReference.fm
Appendix A Software Reference
A-14 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
Example Setup Table Files
Example Code A-15 Relative quantification setup table document
*** SDS Setup File Version 3
*** Output Plate Size 384
*** Output Plate ID 384JQZ55A4
*** Number of Detectors 25
Detector Reporter Quencher Description Comments
18S-VIC-mgb VIC
Bcl-2 FAM Human Bcl-2
Bcl-xL FAM Human Bcl-xL
GAPDH FAM Human GAPDH
GBP2 FAM guanlylate binding protein 2
GOS2 FAM Human GOS2 protein gene
IL-15 FAM Human IL-15
IL-17 FAM Human IL-17
IL-18 FAM Human IL-18
IL-1a FAM Human IL-1a
IL-1b FAM Human IL-1 beta
IL12p40 FAM Human IL-12p40
INF-a2 FAM Interferon-alpha2
IP-10 FAM Human IP-10
ISG56 FAM Interferon-induced protein 56
Nfkb-I FAM Nuclear factor of kappa light...
PKCi FAM Protein kinase C, iota
RAP1A FAM RAP1A, member of RAS oncogene...
RhoPK2 FAM Homo sapiens Rho-associated...
TNF-a FAM Human TNF-alpha
c-fos FAM Human c-fos
c-jun FAM Human c-jun
c-myc FAM Human c-myc
cot FAM Cancer Osaka thyroid oncogene
p53 FAM Human p53
Well Sample Name Detector Task Quantity
1 A1 18S-VIC-mgb ENDO 0.0
2 Liver 18S-VIC-mgb ENDO 0.0 GOS2 TARG 0.0
3 Liver 18S-VIC-mgb ENDO 0.0 cot TARG 0.0
4 Liver 18S-VIC-mgb ENDO 0.0 cot TARG 0.0
5 Liver 18S-VIC-mgb ENDO 0.0 ISG56 TARG 0.0
6 Liver 18S-VIC-mgb ENDO 0.0 ISG56 TARG 0.0
7 Liver 18S-VIC-mgb ENDO 0.0 GOS2 TARG 0.0
8 Liver 18S-VIC-mgb ENDO 0.0 cot TARG 0.0
9 Liver 18S-VIC-mgb ENDO 0.0 cot TARG 0.0
10 Liver 18S-VIC-mgb ENDO 0.0 ISG56 TARG 0.0
11 Liver 18S-VIC-mgb ENDO 0.0 ISG56 TARG 0.0
12 Liver 18S-VIC-mgb ENDO 0.0 GOS2 TARG 0.0
13 Liver 18S-VIC-mgb ENDO 0.0 cot TARG 0.0
14 Liver 18S-VIC-mgb ENDO 0.0 cot TARG 0.0
15 Liver 18S-VIC-mgb ENDO 0.0 ISG56 TARG 0.0
16 Liver 18S-VIC-mgb ENDO 0.0 ISG56 TARG 0.0
...
381 Liver 18S-VIC-mgb ENDO 0.0 GOS2 TARG 0.0
382 Liver 18S-VIC-mgb ENDO 0.0 cot TARG 0.0
383 Liver 18S-VIC-mgb ENDO 0.0 cot TARG 0.0
384 Liver 18S-VIC-mgb ENDO 0.0 ISG56 TARG 0.0
Information for wells 17 through 380 has been removed from this example for brevity.