Applied Biosystems 7900HT User Manual

DRAFT
September 1, 2004 11:39 am, App_SWReference.fm
Setup Table Files
Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide A-13
Format
(for a single well):
[ Well number <tab> SDS Sample Name <tab> Marker name <tab> Marker task ]
{ <tab> Marker name <tab> Marker task <tab> … } [ <cr> ]
Example
Example Code A-12 Allelic discrimination setup table files
*** SDS Setup File Version 3
*** Output Plate Size 384
*** Output Plate ID 384N75822034
*** Number of Detectors 2
Detector Reporter Quencher Description Comments
CYP 2C9*2.1 FAM PDAR CYP 2C9*2 Al1 Example Probe
CYP 2C9*2.2 VIC PDAR CYP 2C9*2 Al2 Example Probe
*** Number of Markers 1
Marker AlleleX AlleleY Description Comments
CYP 2C9*2 CYP 2C9*2.2 CYP 2C9*2.2 PDAR CYP 2C9*2 Example marker
Well Sample Name Detector Task Quantity
1 Sample 1
2 Sample 2
3 ...
384 Sample 48
Well Sample Name Marker Task Marker Task
1 Sample 1 CYP 2C9*2 UNKN
2 Sample 2 CYP 2C9*2 UNKN
3 ...
384 Sample 48 CYP 2C9*2 UNKN
Example Code A-13 Absolute quantification setup table files
*** SDS Setup File Version 3
*** Output Plate Size 96
*** Output Plate ID 96JG729FF903
*** Number of Detectors 1
Detector Reporter Quencher Description Comments
RNase P FAM TAMRA RNase P Probe Example Probe
Well Sample Name Detector Task Quantity Detector Task Quantity
1 Sample 1 GAPDH UNKN 0
2 Sample 2 GAPDH STND 20000
3 ...
96 Sample 30 GAPDH UNKN 0
Example Code A-14 Relative quantification setup table files
*** SDS Setup File Version 3
*** Output Plate Size 96
*** Output Plate ID 96FRE505SDA2
*** Number of Detectors 2
Detector Reporter Quencher Description Comments
GAPDH VIC GAPDH Probe Example Probe
TNF-α FAM TNF-α Probe Example Probe
Well Sample Name Detector Task Quantity Detector Task Quantity
1 Calibrator GAPDH ENDO 0 TNF-α UNKN 0
2 Sample 1 GAPDH ENDO 0 TNF-α UNKN 0
3 ...
96 Sample 12 GAPDH ENDO 0 TNF-α UNKN 0

Applied Biosystems 7900HT Specifications

General

Brand	Applied Biosystems
Model	7900HT
Category	Laboratory Equipment
Language	English