Applied Biosystems 7900HT User Manual

DRAFT
September 1, 2004 11:39 am, CH_Run.fm
Chapter 3 Preparing a Run
3-16 Applied Biosystems 7900HT Fast Real-Time PCR System and SDS Enterprise Database User Guide
Step 3 – Configuring the Plate Document with Tasks
User Access
Requirement
If using the SDS Enterprise Database, you must belong to the Scientist or
Administrator User Group to apply or modify detector task settings.
About Detector
Tasks
You must assign a ‘task’ to the detectors or markers applied to each well of the plate
document that defines their specific purpose or function on the plate. The SDS
software uses the detector task assignments to determine how to treat the data
produced by the wells when analyzing the run data. Detector tasks vary depending on
the type plate document.
Applying
Detector Tasks
1. Using the Ctrl and Shift keys, select the wells of the plate grid containing
samples for a particular task described in the table below.
2. In the well inspector, click the field in the Task column for each detector entry,
and select the appropriate task from the drop-down list. The SDS software labels
all selected wells with the task.
Table 3-1 Detector Tasks of the SDS Software
Experiment Task Apply to…
Allelic
Discrimination
Unknown
all detectors of wells that contain PCR reagents
and test samples.
NTC all detectors of negative control wells that contain
reagents for the PCR, but lack template.
Absolute
Quantification
Unknown all detectors of wells containing PCR reagents and
test samples for quantification.
Standard the appropriate detectors of wells that contain
PCR reagents and samples of known quantities.
(See page 3-17 for instructions.)
NTC all detectors of negative control wells that contain
PCR reagents, but lack template.
Relative
Quantification
Targ e t all detectors of wells that contain PCR reagents for
the amplification of target sequences.
Endogenous
Control
all detectors of wells that contain reagents for the
amplification of the endogenous control sequence.
Task drop-down list
Selected wells
(task applied
to selection)

Applied Biosystems 7900HT Specifications

General

Brand	Applied Biosystems
Model	7900HT
Category	Laboratory Equipment
Language	English